A SINGLE‐CELL ATLAS OF DIFFUSE LARGE B CELL LYMPHOMA

Diffuse large B-cell lymphoma (DLBCL) is one of the most common yet aggressive types and remains incurable in 40% patients. Herein, we profiled transcriptomes 94,324 cells from 17 DLBCLs 3 control samples using single-cell RNA-sequencing technology, creating a comprehensive map for tumor infiltrating immune DLBCL. High degrees inter- intratumor heterogeneity were revealed, identified 73 gene expression programs expressed malignant B cells. We further characterized 8 nonmalignant subclusters 16 T-cell microenvironment. Six myeloid also identified, including LAMP3+ DC subcluster enriched ABC-DLBCL. More than 2000 likely cell-cell interactions predicted DLBCL, illustrating complex highly dynamic DLBCL Unique to cell lymphomas, strong costimulatory CD70-CD27 interaction was between T Furthermore, coinhibitory signals mediated by TIM-3 or TIGIT seemed be main driving force exhaustion. Finally, discovered that chronic hepatitis virus infection may have significant impact on survival evasion Our results provide new insights into lymphomagenesis facilitate design targeted immunotherapy strategies. The research funded by: Swedish Cancer Society, STINT, CIMED, Radiumhemmet, Research Council, Knut Alice Wallenberg Foundation, Guangdong Enterprise Key Laboratory Human Disease Genomics, Shenzhen Single-Cell Omics Keywords: non-Hodgkin lymphoma, genomics, epigenomics, other -omics, biology No conflicts interests pertinent abstract.